Molecular biology has revolutionized research into vascular disease.
Over the past 20 years molecular techniques have enabled us to both
elucidate - lecular mechanisms in vascular disease and identify
appropriate therapies. The vast explosion in technical knowledge and the
array of protocols that become more advanced and intricate by the day
lead us into new and exciting areas of research that were previously
unobtainable. Vascular Disease: Molecular Biology and Gene Transfer
Protocols - scribes today's most powerful molecular methods for the
investigation of the pathogenesis of vascular disease. The protocols are
highly detailed, allowing beginners who have little experience in either
vascular biology or molecular biology to embark on new molecular
projects. This book is also suited to more experienced molecular
biologists who wish to grasp new methods for stu- ing the involvement of
genes in normal vascular physiology and in diseased states. It is well
established that cardiovascular disease progression has a s- stantial
genetic influence. Part I describes three methods that have been used
successfully to identify specific mutations in candidate genes involved
in c- diovascular disorders. These mutations include both
single-stranded conf- mational polymorphism analysis and heteroduplex
detection methods. In addition, technology to map new genes to specific
regions of chromosomes by high-resolution mapping is described.
