Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in Ghana
and Africa as a whole. In this study, 30 accessions of Solanum
lycopersicum L. with reported TYLCV- resistance from AVRDC were assessed
for resistance to TYLCV in Ghana. Plants were grown in a field, a hot
spot of the disease and the reactions of plants were evaluated based on
the disease symptoms when they were 30, 45 and 60 days after
transplanting. Molecular screening was also done to re-confirm the
phenotypic evaluation. All the tomato accessions demonstrated various
degrees of disease symptoms. Phenotypic evaluation was confirmed by
amplification of TYLCV DNA fragment in all tested accessions. Based on
both the phenotypic and molecular evaluations, no accession provided
complete resistance to TYLCV in Ghana. However accessions with milder
symptoms of TYLCV in the field but no viral DNA were assessed as
tolerant.