John R. Crowther provides today's premier practical guide to the
understanding and application of ELISA. Updating and greatly expanding
his widely appreciated earlier publication, ELISA Theory and Practice
(1995), this important work introduces chapters on such major new topics
as checkerboard titrations, quality control of testing, kit production
and control, novel monoclonal antibodies, validation of assays,
statistical requirements for data examination, and epidemiological
considerations. With its numerous worked examples, detailed
instructions, and extensive illustrations, The ELISA Guidebook offers a
powerful synthesis of all the basic concepts and practical experimental
details investigators need to understand, develop, and apply the new
ELISA methodology successfully in day-to-day basic and clinical
research.