In plant biotechnology, the in-vitro studies are performed by culturing
the plant cell, tissue and organ on an artificially prepared nutrient
medium static or liquid under aseptic conditions. The effect of some
plant growth regulators were studied on seedling growth and stomatal
characters of Brassica campestris L. V.mungo (L.) Hepper, V.radiate (L.)
Wilczek.) through in-vitro studies. Black gram (Vigna mungo), is an
important short duration pulse crop grown in many parts of India,
cultivating both in Kharif and Rabi season. The optimum temperature for
better growth of these crops ranges between 25 to 350C, but it can
tolerate up to 420C which permit to cultivate during summer and winter
season. Mungbean crop (Vigna radiata (L) Wilczek) is considered as one
of the new crop in Egypt, it is important legume crop characterized by a
relative high content of protein. The application of novel tissue
culture and genetic engineering technology could offer efficient and
stable plant regeneration. Brassica are still considered as an important
source of green vegetable in the developing countries and the potential
for market diversification of some new crop types.