Most forest tree species were considered recalcitrant a decade ago, but
now with the improved in vitro techniques some progress has been made
towards culture-of tree species. Micro- propagation has been achieved
from the juvenile tissues of a number of forest tree species. On the
other hand, tissues from most mature trees are still very difficult to
grow and differen- tiate in vitro. Nevertheless, there has been slow but
steady progress in the application of tissue culture technology for
culture of tissues, organs, cells and protoplasts of tree species. As
compared to most agricultural crops, and herbaceous plant species, trees
are a different lot. They have long gene- ration cycles. They are highly
heterozygous and have a large reservoir of genetic variability. Because
of this genetic variability, their response in vitro is also variable.
On a single medium, the response of tissues from different trees
(genotypes) of a single species may be quite different: some responding
by induction of growth and differentiation, while others showing minimal
or no growth at all. That makes the somatic cell genetics of woody
plants somewhat difficult, but at the same time interesting.