In the last three decades, the fast development of single-molecule
techniques has revolutionized the way we observe and understand
biological processes. Some of these techniques have been further adapted
as tools for bioanalysis. This book summarizes and details the frontiers
of the development of these tools as well as their applications. The
contributors are young and established researchers in their respective
fields. The main content originates from the lecture notes of a
chemistry graduate course taught by the book editor at Nanjing
University. This book is suitable to be used as a textbook for a
high-level undergraduate or an entry-level graduate course. The
systematically written content provides a thorough illustration of the
mechanisms of each methodology presented.