Salmonella: Methods and Protocols, Second Edition expands upon the
previous edition with current, detailed methods on different aspects and
landmarks advancing knowledge on salmonella research. With new chapters
on molecular assays for detection, identification and serotyping of
salmonella, quantitative proteomic identification of host factors
involved in salmonella infection, determination of antimicrobial
resistance in salmonella, site-directed mutagenesis, chromosomal gene
analysis, development of bacterial nanoparticle vaccine, attachment of
nanoparticle cargo to biotinylated salmonella for combination
bacteriotherapy against tumors, various microscopy methods to analyze
salmonella interaction with host cells, in vitro modeling of
gallbladder-associated salmonella colonization, and analysis of
salmonella phages and prophages. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Salmonella: Methods and Protocols,
Second Edition present methods that are of value to investigators in
the salmonella field.