In RNA Mapping- Methods and Protocols expert researchers in the field
detail many of the methods which are now commonly used to study RNA.
These include protocols for the consequence of the emerging interest in
the characterization of cellular RNAs urged by their potential use as
diagnostic biomarkers or therapeutic targets. In particular, the
biological relevance of microRNAs in human physiology and disease
development is highlighted in the 16 chapters focused on methods for
their physical and functional mapping. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Mapping- Methods and Protocols
provides instruction and inspiration for scientists who are facing the
challenges of the discovery and/or functional characterization of RNA
molecules for a wide variety of applications ranging from novel
biomedical diagnostics to therapeutics and biomaterials.