This study was conducted to investigate the influence of ACC deaminase
rhizobacteria on the ACC-induced classical "triple" response in
etiolated pea seedlings. Etiolated pea seedlings were exposed to
different concentrations of ACC (0, 2, through 10 mmol L-1), in 100 ml
glass beakers placed in airtight mason jars wrapped in green foil and
incubated under dark for seven days. In another study pea seedlings were
inoculated with five strains of rhizobacteria which vary in their
ACC-deaminase activity. These inoculated pea seedlings were exposed to
10 mmol L-1 ACC and incubated in the darkness at 25 ± 3 °C. Results
revealed that exogenous application of ACC had a concentration-dependent
effect in creating classical "triple" response in etiolated pea
seedlings. Our study concludes that the inoculation with rhizobacteria
containing ACC deaminase could be used to decrease ACC which produces
due to a variety of biotic and abiotic stresses in plants.