This manual reflects practical approaches to handling bacteria in the
labora tory. It is designed to recall historical methods of bacterial
genetics that have had recent developments and to present new techniques
that allow full genome analysis. It has been written for microbiologists
who need to group their protocols at the state of the art of a new
millennium and also for scientists in other fields of life sciences who
need to use bacteria for their research. Teachers, graduate students,
and postdocs also will benefit from having these protocols to help them
understand modern bacterial genetics. I learned so much from these
contributions from my colleagues that I have no doubt about the daily
usefulness of this book. April 2002 Michel Blot XII Abbreviations
Acyl-HSL N-acyl homoserine lactone moi multiplicity of infection Amp or
Ap ampicillin N amino C carboxy NMR nuclear magnetic resonance CIO-HSL
N-decanoyl-L-homoserine lactone 3-0H-C14:1-HSL N-(3-hydroxy-7
-cis-tetra- C12-HSL N-dodecanoyl-L-homoserine lac- decanoyl)homo-serine
lactone tone 3-0H-C4-HSL N-3-hydroxybutanoyl-L- C14-HSL
N-tetradecanoyl-L-homoserine homoserine lactone lactone ONPG
o-nitrophenyl -D-galactopyranoside C4-HSL N-butanoyl-L-homoserine
lactone ORF open reading frame C6-HSL N-hexanoyl-L-homoserine lactone
OTG I-S-octyl- -D-thioglucoside C8-HSL N-octanoyl-L-homoserine lactone
3-oxo-CIO-HSL N-3-oxodecanoyl-L-homo- Cam or Cm chloramphenicol serine
lactone CBD chitin binding domain 3-oxo-C12-HSL N-3-oxododecanoyl-L CHEF
contour clamped homogenous electric homoserine lactone field
3-oxo-C14-HSL N-3-oxotetradecanoyl-L CI consistency index homoserine
lactone CRIM conditional-replication, integration, 3-oxo-C4-HSL
N-3-oxobutanoyl-L-homoser and modular ine lactone dCTP deoxycytidine
triphosphate 3-oxo-C6-HSL N-3 -oxohexanoyl-L-homoser deg."
