Thoroughly revised and updated, Optimization in Drug Discovery: In
Vitro Methods, Second Edition presents a wide spectrum of in vitro
assays including formulation, plasma binding, absorption and
permeability, cytochrome P450 (CYP) and UDP-glucuronosyltransferases
(UGT) metabolism, CYP inhibition and induction, drug transporters,
drug-drug interactions via assessment of reactive metabolites,
genotoxicity, and chemical and photo-mutagenicity assays. Written for
the Methods in Pharmacology and Toxicology series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible protocols,
and tips on troubleshooting and avoiding known pitfalls. Expert authors
have developed and utilized these in vitro assays to achieve "drug-like"
characteristics in addition to efficacy properties and good safety
profiles of drug candidates.
Comprehensive and up-to-date, Optimization in Drug Discovery: In Vitro
Methods, Second Edition aims to guide researchers down the difficult
path to successful drug discovery and development.