The application of optical methods for investigating neocortical circuit
dynamics has greatly expanded in recent years, providing novel insights
into the fascinating world of brain function. Optical Imaging of
Neocortical Dynamics presents a guide to these indispensible tools,
which cover a broad range of spatiotemporal scales and a large variety
of signaling aspects, ranging from voltage changes to metabolic states.
This detailed volume specifically explores methods that are applied in
experiments on living brains (in vivo) and that relate to functional
properties on the spatial scale of cortical circuits. Beginning with an
introductory section that focuses on physical fundamentals of optical
imaging as well as molecular tools used for in vivo optical imaging and
optogenetic control, the book continues with the most relevant methods
and their applications to investigate changes in neuronal and glial
activity states as well as optical imaging methods probing metabolic
states. Written for the Neuromethods series, this volume contains the
kind of detail and key implementation advice that ensures successful
results in the lab.
Practical and easy to use, Optical Imaging of Neocortical Dynamics
serves as an ideal guide for researchers who aim to apply these highly
valuable tools to their own neuroscientific studies.