This study aims to evaluate oxidative stress status and investigate some
important genes that were related to type 2 diabetes mellitus. This
study was a case-control study conducted from November 2015 to April
2016 and included 60 Iraqi patients with diabetes mellitus type 2 (35
females and 25 males) with an average age (40-60 year), and it was
carried out at diabetes and endocrine care center Al-Sadder Teaching
Hospital, Al-Najaf, Iraq. Also, this study included 40 of apparently
healthy people who were (20 females and 20 males) with an average age
(40-60) as control matched with disease groups. The present study was
divided into two main parts: biochemical and molecular. The biochemical
part included study total antioxidants status (TAS), reactive oxygen
species (ROS) levels and fasting blood glucose (FBG) levels, while
molecular part included study catalase gene by using polymerase chain
reaction technique (PCR) and restriction fragment length polymerase
(RFLP-PCR), and GSTM1 and GSTT1 genes by using Multiplex technique.