This manual describes the methodology and applications of
microinjection, a powerful technique for transposing material into
living cells through needle injection. Information is presented on the
techniques principles, the required equipment, preparation of receiving
cells and injected material, and the analysis of results. Detailed
protocols for a wide range of applications in different cellular systems
are included. A large part of the book is also dedicated to the Xenopus
laevis oocyte system.