We continue in this second volume the plan evident in the first; i.e.,
of presenting a number of well-rounded up-to-date reviews of important
developments in the exciting field of ion-selective electrodes in
analytical chemistry. In this volume, in addition to the exciting
applications of ISE'S to biochemistry systems represented by the
description of enzyme electrodes, there is featured the most recent
development in ISE'S, namely, the joining of the electrochemical and
solid state expertise, resulting in CHEMFETS. The scholarly survey of
the current status of ISE'S will undoubtedly be welcomed by all workers
in the field. Tucson, Arizona Henry Freiser vii Contents Chapter 1
Potentiometric Enzyme Methods Robert K. Kobos 1. Introduction . . . . .
. 1 2. Soluble Enzyme Systems . . . 5 2.1. Substrate Determinations 5
2.2. Enzyme Determinations . 13 2.3. Inhibitor Determinations. 18 3.
Immobilized Enzyme Systems . 19 3.1. Methods of Immobilization. 19 3.2.
Characteristics of Immobilized Enzymes 23 3.3. Analytical Applications
with Ion-Selective Electrodes 23 4. Enzyme Electrodes 31 4.1. Urea
Electrodes 35 4.2. Amygdalin Electrodes 39 4.3. Glucose Electrodes . 40
4.4. Penicillin Electrodes 40 4.5. Amino Acid Electrodes 41 4.6.
Nucleotide Electrodes 46 4.7. Uric Acid Electrode 47 4.8. Creatinine
Electrode 48 48 4.9. Acetylcholine Electrodes. 4.10. D-Gluconate
Electrode 49 4.11. Lactate Electrode 49 4.12. Inhibitor Determination 50
4.13. Substrate Electrodes 50 4.14. Current Trends . . . .