This Brief highlights different approaches used to create stable
cellulase and its use in different fields. Cellulase is an industrial
enzyme with a broad range of significant applications in biofuel
production and cellulosic waste management. Cellulase 7a from
Trichoderma reesei is the most efficient enzyme in the bio hydrolysis of
cellulose. In order to improve its thermal stability, it can be
engineered using a variety of approaches, such as hydrophobic
interactions, aromatic interactions, hydrogen bonds, ion pairs and
disulfide bridge creation.