Discover how to use HILIC to analyze and better understand polar
compounds
An increasingly popular analytical method, hydrophilic interaction
chromatography (HILIC) has the ability to retain and separate polar
compounds that are often difficult to analyze by reversed-phase
high-performance liquid chromatography (HPLC) or other analytical
methods. Offering a comprehensive review, this book enables readers to
develop a fundamental understanding of how HILIC works and then apply
that knowledge to develop and implement a variety of practical
applications.
Hydrophilic Interaction Chromatography begins with discussions of
HILIC retention mechanisms, stationary phases, and general method
development. This sets the foundation for the book's extensive coverage
of applications. The authors address unique separation challenges for
bioanalytical, environmental, pharmaceutical, and biochemical
applications. Moreover, there is a thorough discussion of HILIC in
two-dimensional chromatography.
With contributions from leading analytical scientists who have extensive
experience in HILIC as well as HPLC, Hydrophilic Interaction
Chromatography serves as a practical guide for researchers, featuring:
- Detailed examples of HILIC methods and development approaches
- Thorough explanations of retention mechanisms and the impact of
stationary phase and mobile phase properties on separations
- Step-by-step guidance for developing efficient, sensitive, and robust
HILIC methods
- References to the primary literature at the end of each chapter
Hydrophilic Interaction Chromatography is written for scientists who
use or develop analytical methods for the separation of polar compounds.
In particular, these researchers will discover how HILIC can be used to
analyze and better understand the composition of pharmaceutical,
bioanalytical, biochemical, chemical, food, and environmental samples.
