Master's Thesis from the year 2012 in the subject Pharmacology, grade:
A, Mahatma Gandhi University (University College of Pharmacy), course:
M.Pharm, language: English, abstract: Polyherbal formulations available
with a wide range of indications like hepatoprotective, appetite and
growth promoters, gastrointestinal and hepatic regulator, as treatment
for hepatic dysfunction, for hepatic regeneration as well as liver
stimulant and tonic. Despite the widespread use, there is a lack of
scientific evidence on their efficacy and safety. This study was
designed to evaluate the effect of Polyherbal formulation Kamialri on
oxidative stress and associated damages in carbon tetrachloride induced
toxicity in Chang liver cells. The study is mainly focused on the
evaluation of its antioxidant potential, invitro steatosis and
intracellular localisation of reduced glutathione by
Monochlorobimane(MCB) staining. Carbon tetrachloride induced toxicity in
chang liver cells was well manifested by significant decrease in cell
viability, enhanced lipid peroxidation leading to tissue damage,
decreased SOD, glutathione, glutathione peroxidase and glutathione S-
transferase and increased deposition of fatty acid . MTT study revealed
that Kamilari has got cytoprotective activity. It has got the excellent
free radical scavenging properties which was proven by various
antioxidant activities (LP, SOD, GSH, GPX, and GST). Inhibitory effect
of Kamilari on fatty acid deposition was proven by invitro steatosis
studies which were conducted on palmitic acid treated chang cell
line.Based on the above studies it is concluded that Kamilari has
cytoprotective and antioxidant activity, and found to be effective in
steatosis.
