Genetic diversity was assessed using 15 morphological characters. Based
on multivariate (D2) analysis, 60 mango genotypes were grouped into 8
clusters. Three enzymes viz., gulutamate oxaloacetate transminase (GOT),
malate dehydrogenase (MDH) and peroxidase (PER) were used to investigate
genetic diversity of 60 mango genotypes at protein level. Eight
zymotypes with GOT, 10 with MDH and 7 with PER were formed by 22, 39, 12
bands, respectively at different Rf values. Compared with three studied
DNA extraction protocols of mango such as SDS, CTAB and water saturated
ether (WSE) method with NaCl, it was found that WSE method with NaCl had
highest value of average percentage (85.44%) in DNA content of the mango
genotypes. Study of RAPD for 60 mango genotypes was done to detect
genetic diversity at DNA level. Of 40 primers, 11 decamer primers
amplified 104 bands. The mean genetic diversity among all the accessions
was 0.323 and Shannon's information index was 0.489 across all loci.
Induction of mutation on 8 selected genotypes derived from
morph-molecular diversity study was performed by applying three doses of
radiation such as 20 Gy, 30 Gy and 40 Gy.