This manual offers detailed protocols for fluorescence in situ
hybridization (FISH) and comparative genomic hybridization approaches,
which have been successfully used to study various aspects of genomic
behavior and alterations. Methods using different probe and cell types,
tissues and organisms, such as mammalians, fish, amphibians (including
lampbrush-chromosomes), insects, plants and microorganisms are described
in 57 chapters. In addition to multicolor FISH procedures and special
applications such as the characterization of marker chromosomes,
breakpoints, cryptic aberrations, nuclear architectures and epigenetic
changes, as well as comparative genomic hybridization studies, this 2nd
edition describes how FISH can be combined with other techniques. The
latter include immunostaining, electron microscopy, single cell
electrophoresis and microdissection.
This well-received application guide provides essential protocols for
beginning FISHers and FISH experts alike working in the fields of human
genetics, microbiology, animal and plant sciences.