Focused on one of the most important and fascinating fields of modern
biology, Exocytosis and Endocytosis, Second Edition presents a
comprehensive collection of protocols for cutting edge, recently
developed methods of studying exocytosis and endocytosis in vitro and
in vivo. Divided into four easy-to-follow sections, chapters focus on
diverse research topics including cell-free and biochemical assays of
exocytosis and endocytosis, probing protein, membrane and organelle
trafficking in cultured cells and powerful in vivo imaging approaches
to investigate different physiological processes involving exocytosis
and endocytosis. Written in the successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls.
Highly practical and clearly written, Exocytosis and Endocytosis,
Second Edition will serve both seasoned researchers as well as
newcomers to the field and will provide a unique resource and expert
guidance to modern laboratory techniques developed for examining protein
and membrane trafficking in simple cellular systems and model organisms.