Irshad Ahmad

(Author)

Enhancement of Xylitol Production in Candida TropicalisPaperback, 3 January 2012

Enhancement of Xylitol Production in Candida Tropicalis
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Print Length
108 pages
Language
English
Publisher
LAP Lambert Academic Publishing
Date Published
3 Jan 2012
ISBN-10
3847335014
ISBN-13
9783847335016

Description

In this study, gk gene was disrupted from xylitol dehydrogenase gene (XYL2) knockout C. tropicalis strain BSXDH-3. The resulting strain was incapable to grow on glycerol. The cells growth on glycerol was resumed by co-expression of gcy1, 2 and 3 genes from Scheffersomyces stipitis under the control of C. tropicalis glyceraldehydes-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "IA-3", than in BSXDH-3. The pentose phosphate pathway (PPP) is a major basis of NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes, which respectively encode glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. This is the first report of increased metabolic flux toward glycerol metabolic and PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production.

Product Details

Author:
Irshad Ahmad
Book Format:
Paperback
Country of Origin:
US
Date Published:
3 January 2012
Dimensions:
22.86 x 15.24 x 0.66 cm
ISBN-10:
3847335014
ISBN-13:
9783847335016
Language:
English
Location:
Saarbrucken
Pages:
108
Weight:
167.83 gm

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