Electron Probe Microanalysis: Applications in Biology and Medicine (Softcover Reprint of the Original 1st 1989)Paperback - Softcover Reprint of the Original 1st 1989, 16 December 2011

Electron Probe Microanalysis: Applications in Biology and Medicine (Softcover Reprint of the Original 1st 1989)
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Part of Series
Springer Biophysics
Part of Series
Springer Series in Biophysics
Print Length
322 pages
Language
English
Publisher
Springer
Date Published
16 Dec 2011
ISBN-10
3642744796
ISBN-13
9783642744792

Description

The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti- cular diffusible ions (elements). 2. Electron probe microanaly- sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con- verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo- gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.

Product Details

Book Edition:
Softcover Reprint of the Original 1st 1989
Book Format:
Paperback
Country of Origin:
NL
Date Published:
16 December 2011
Dimensions:
23.39 x 15.6 x 1.8 cm
ISBN-10:
3642744796
ISBN-13:
9783642744792
Language:
English
Location:
Berlin, Heidelberg
Pages:
322
Publisher:
Springer
Weight:
476.27 gm

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