Directed Evolution Library Creation: Methods and Protocols, Second
Edition presents user-friendly protocols for both proven strategies and
cutting-edge approaches for the creation of mutant gene libraries for
directed evolution. As well as experimental methods, information on
current computational approaches is provided in a user-friendly format
that will allow researchers to make informed choices without needing to
comprehend the full technical details of each algorithm. Directed
evolution has become a fundamental approach for engineering proteins to
enhance activity and explore structure-function relationships, and has
supported the rapid development of the field of synthetic biology over
the last decade. Divided into three convenient sections, topics include
point mutagenesis strategies, recombinatorial methods wherein genetic
diversity is sourced from multiple parental genes that are combined via
either homology-dependent or -independent techniques and a variety of
computational methods to guide the design and analysis of mutant
libraries. Written in the successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols and notes on troubleshooting and avoiding
known pitfalls.
Authoritative and easily accessible, Directed Evolution Library
Creation: Methods and Protocols, Second Edition will serve as a
reliable manual for both novice and experienced protein engineers and
synthetic biologists and will enable further technical innovation and
the exploitation of directed evolution for a deeper understanding of
protein design and function.
