This book presents various examples of how advanced fluorescence and
spectroscopic analytical methods can be used in combination with
computer data processing to address different biochemical questions. The
main focus is on evolutionary biochemistry and the description of
biochemical and metabolic issues; specifically, the use of pulse
amplitude modulated fluorescence (PAM) for the functional analysis of
the cellular state, as well as results obtained by means of the
derivative spectroscopy method characterizing structural reorganization
of a cell under the influence of external factors, are discussed. The
topics presented here will be of interest to biologists, geneticists,
biophysicists and biochemists, as well as experts in analytical
chemistry, pharmaceutical chemistry and radio chemistry and radio
activation studies with protonen and alpha-particles. It also offers a
valuable resource for advanced undergraduate and graduate students in
biological, physical and chemical disciplines whose work involves
derivative spectrophotometry and PAM-fluorescence.