In the ten years since the publication of the first edition, great
advances in fluorescent labeling, optics, and sample preparation have
significantly improved the imaging capability of microscopy, allowing
for a continual refinement of our understanding of the cytoskeleton as a
dynamic synergy of components. In Cytoskeleton Methods and Protocols,
Second Edition, internationally renowned experts present techniques
which reflect many of the recent technological advances in experimental
tools for cytoskeleton research with emphasis on animal, plant, protist,
and fungal model systems. This cutting-edge volume contains methods for
live-cell imaging, fluorescence microscopy, electron microscopy,
analysis of cell and organelle motility, isolation of cytoskeleton
components, and proteomics, amongst other topics. As a volume in the
highly successful Methods in Molecular Biology(TM) series, chapters
incorporate introductions to their respective subjects, lists of the
necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes that provide unpublished technical
information on troubleshooting and avoiding known pitfalls.
Up-to-date and comprehensive, Cytoskeleton Methods and Protocols,
Second Edition serves as an ideal guide to scientists who wish to
continue this fruitful and important biological research.
