The current demand for the development of techniques for controlled
genetic manipulations is driven by the anatomical and physiological
complexity of the brain and by the need for experimental models that can
address this complexity through selective manipulation of defined
components of the system: specific neuronal populations or selected
synapses. In Controlled Genetic Manipulations, expert researchers
present detailed chapters to supply basic technical information about
controlled genetic manipulations and to provide examples of creative
implementation of this methodology when addressing a unique biological
problem. Some chapters of the book describe the most recent developments
in the basic methodology, which includes use of Cre-recombinase, methods
for delivery of genetic material into the brain and the use of
optogenetics, whereas other chapters focus on applying these techniques
to addressing particular biological questions like structural and
functional mapping of neuronal circuits, analysis of specific synaptic
connections, modeling or gene therapy of neurological disorders. As part
of the Neuromethods series, chapters include key implementation advice
that is crucial for getting optimal results.
Authoritative and practical, Controlled Genetic Manipulations serves
as a valuable guide to a variety of techniques, provided by many of the
pioneers of the approaches.
