Blood-brain barrier (BBB) breakdown leading to cerebral edema occurs in
many brain diseases--such as trauma, stroke, inflammation, infection,
and tumors--and is an important factor in the mortality arising from
these con- tions. Despite the importance of the BBB in the pathogenesis
of these diseases, the molecular mechanisms occurring at the BBB are not
completely und- stood. In the last decade a number of molecules have
been identified not only in endothelial cells, but also in astrocytes,
pericytes, and the perivascular cells that interact with endothelium to
maintain cerebral homeostasis. However, the precise cellular
interactions at a molecular level in steady states and d- eases have
still to be determined. The introduction of new research techniques
during the last decade or so provide an opportunity to study the
molecular mec- nisms occurring at the BBB in diseases. The Blood-Brain
Barrier: Biology and Research Protocols provides the reader with details
of selected morphologic, permeability, transport, in vitro, and
molecular techniques for BBB studies, all written by experts in the
field. Each part is preceded by a review that emphasizes the advantages
and pitfalls of particular techniques, as well as offering much relevant
current information. The techniques provided will be helpful to both
beginners in BBB research and those more experienced investigators who
wish to add a specific technique to those already available in their
laboratories.
