The demand for chemical free food products is increasing rapidly over
the globe and biotechnology can play a significant role to meet the
purpose. The enzymes extracted from microbial sources are being deployed
to get bakery items of high quality and safety. Present research was
conducted to synthesize xylanase enzyme by Aspergillus niger using local
agricultural wastes for its utilization in baking industry. Wheat bran,
corn cobs and sugar cane bagasse were used as carbon source employing
submerged fermentation. Wheat bran showed maximum xylanase activity
followed by corn cob and sugar cane bagasse. As the ultimate goal was to
apply xylanase in bakery products i.e. bread, so it was subjected to
efficacy studies. It is deduced that values for body growth performance,
serological and hematological parameters were within normal ranges
showing the safety of enzyme. In order to assess the potential of
xylanase in bread making; a comparative study was carried out which
concluded that the xylanase addition modified the rheological attributes
of dough resulting the increased bread volume. Lastly, sensory
evaluation showed marked improvement in organoleptic properties of
bread.