This comprehensive and unique text presents a full overview of
downstream processing useful for those new to the concept as well as
professionals with experience in the area. The history and theoretical
principles of Aqueous Two-Phase Systems (ATPS) are covered in depth.
Information on ATPS characterization and application is included, and
ATPS equilibria and system parameters that have significant effect on
partition behavior are studied.
Aqueous Two-Phase Systems for Bioprocess Development for the Recovery
of Biological Products addresses specific applications of ATPS for the
recovery and partial purification of high molecular weight compounds
such as proteins, nucleic acids and polysaccharides, particulate
bioproducts such as cells and organelles and low molecular weight
compounds. Non-conventional strategies involving ATPS such as affinity
systems, continuous liquid-liquid fractionation stages and the recovery
from plant extracts are presented. Economic analysis of the application
of ATPS in comparison to other fractionation techniques, particularly
liquid chromatography, is considered, as are opportunity and current
trends in the ATPS research area.
Each chapter utilizes the contributors' experimental expertise in
traditional and non-conventional ATPS strategies, as well as analysis of
areas of opportunity and perspectives on the development and future
applications of ATPS in both the lab and larger scale operations. The
result is a thorough and singular overview of ATPS which has not been
matched by any other text on the market.
