Animal Cell Biotechnology: Methods and Protocols, Second Edition
constitutes a comprehensive manual of state-of-the-art and new
techniques for setting up mammalian cell lines for production of
biopharmaceuticals, and for optimizing critical parameters for cell
culture considering the whole cascade from lab to final production. The
volume is divided into five parts that reflect the processes required
for different stages of production. In Part I, basic techniques for
establishment of production cell lines are addressed, especially
transduction techniques, cells for gene therapy and antibody production.
Part II addresses basic cultivation techniques, such as microcarrier
culture and encapsulation.
Part III covers cell characterization and analysis, including flow
cytometric applications, NMR-based techniques, and biochemical and
cytometric techniques. Part IV details cultivation techniques, such as
disposable bioreactors, hollow fiber cell culture, fixed bed reactors,
and configuration of bioreactors. Part V covers downstream techniques
such as membrane filtration techniques, while Part VI describes special
applications, including retroviral vectors.
Animal Cell Biotechnology: Methods and Protocols, Second Edition
provides a compendium of techniques for scientists in industrial and
research laboratories that use mammalian cells for biotechnology
purposes.