Amino Acid Analysis Protocols (2000)Hardcover - 2000, 5 September 2000

Amino Acid Analysis Protocols (2000)
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Part of Series
Methods in Molecular Biology
Print Length
265 pages
Language
English
Publisher
Humana
Date Published
5 Sep 2000
ISBN-10
0896036561
ISBN-13
9780896036567

Description

Amino acid analysis is a technique that has become commonplace in biotechnology, biomedical, and food analysis laboratories. This book describes a variety of amino acid analysis techniques and how each technique can be used to answer specific biological questions. The first two chapters in Amino Acid Analysis Protocols introduce the concepts, basic theory, and practice of amino acid analysis. The following chapters give detailed instructions on various methods and their applications. As highlighted, there are many different approaches to amino acid ana- sis, but in all cases the results depend heavily on the quality of the sample. Therefore a new way to desalt samples prior to hydrolysis is covered as an introductory chapter (Chapter 3), and most authors have devoted a section to sample preparation, especially to the collection and storage of bodily fluids. Some of the amino acid analysis methods described in this book are based on HPLC separation and analysis after precolumn derivatization. The precolumn derivatization techniques described use (a) 6-aminoquinolyl-N-hydro- succinimidyl carbamate (AQC) (Chapters 4 and 8); (b) 1-fluoro-2- dinitrophenyl-5-L-alanine amide (Marfey's reagent), which allows sepa- tion and analysis of enantiomeric amino acids (Chapter 5); (c) O-phthalal- hyde (OPA) (Chapters 6 and 10); (d) butylisothiocyanate (BITC) and benzylisothiocyante (BZITC) (Chapter 11); (e) phenylisothiocyanate (PITC) (Chapters 12 and 13); (f) ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-s- fonate (SBD-F) (Chapter 17); and (g) 9-fluorenylmethyl-chloroformate (FMOC-Cl) (Chapter 10).

Product Details

Book Edition:
2000
Book Format:
Hardcover
Country of Origin:
US
Date Published:
5 September 2000
Dimensions:
23.67 x 16 x 2.41 cm
ISBN-10:
0896036561
ISBN-13:
9780896036567
Language:
English
Location:
Totowa, NJ
Pages:
265
Publisher:
Humana
Weight:
580.6 gm

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